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DCEFs improved odontogenic differentiation ability of SCAP in vitro. (a, b) 200 mV/mm DCEFs significantly increased <t>ALP</t> expression of SCAP (scale bar = 200 μm). (c, d) Alizarin red <t>S</t> <t>staining</t> showed that DCEFs could increase mineralised nodule formation with the highest effect at a magnitude of 200 mV/mm (scale bar = 200 μm). (e–k) DCEFs partially recovered the decreased mRNA and protein expression of DSPP, DMP1 and ALP induced by cytoskeletal polymerization inhibitors (NS: p > .05, * p < .05, ** p < .01, *** p < .001).
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DCEFs improved odontogenic differentiation ability of SCAP in vitro. (a, b) 200 mV/mm DCEFs significantly increased <t>ALP</t> expression of SCAP (scale bar = 200 μm). (c, d) Alizarin red <t>S</t> <t>staining</t> showed that DCEFs could increase mineralised nodule formation with the highest effect at a magnitude of 200 mV/mm (scale bar = 200 μm). (e–k) DCEFs partially recovered the decreased mRNA and protein expression of DSPP, DMP1 and ALP induced by cytoskeletal polymerization inhibitors (NS: p > .05, * p < .05, ** p < .01, *** p < .001).
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DCEFs improved odontogenic differentiation ability of SCAP in vitro. (a, b) 200 mV/mm DCEFs significantly increased <t>ALP</t> expression of SCAP (scale bar = 200 μm). (c, d) Alizarin red <t>S</t> <t>staining</t> showed that DCEFs could increase mineralised nodule formation with the highest effect at a magnitude of 200 mV/mm (scale bar = 200 μm). (e–k) DCEFs partially recovered the decreased mRNA and protein expression of DSPP, DMP1 and ALP induced by cytoskeletal polymerization inhibitors (NS: p > .05, * p < .05, ** p < .01, *** p < .001).
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DCEFs improved odontogenic differentiation ability of SCAP in vitro. (a, b) 200 mV/mm DCEFs significantly increased ALP expression of SCAP (scale bar = 200 μm). (c, d) Alizarin red S staining showed that DCEFs could increase mineralised nodule formation with the highest effect at a magnitude of 200 mV/mm (scale bar = 200 μm). (e–k) DCEFs partially recovered the decreased mRNA and protein expression of DSPP, DMP1 and ALP induced by cytoskeletal polymerization inhibitors (NS: p > .05, * p < .05, ** p < .01, *** p < .001).

Journal: International Endodontic Journal

Article Title: Electric field promoted odontogenic differentiation of stem cells from apical papilla by remodelling cytoskeleton

doi: 10.1111/iej.14213

Figure Lengend Snippet: DCEFs improved odontogenic differentiation ability of SCAP in vitro. (a, b) 200 mV/mm DCEFs significantly increased ALP expression of SCAP (scale bar = 200 μm). (c, d) Alizarin red S staining showed that DCEFs could increase mineralised nodule formation with the highest effect at a magnitude of 200 mV/mm (scale bar = 200 μm). (e–k) DCEFs partially recovered the decreased mRNA and protein expression of DSPP, DMP1 and ALP induced by cytoskeletal polymerization inhibitors (NS: p > .05, * p < .05, ** p < .01, *** p < .001).

Article Snippet: ALP staining of SCAP was performed using the ALP colour development kit (Beyotime), and the ALP activity was quantitatively assessed using the ALP assay kit (Beyotime), following the manufacturer's instructions.

Techniques: In Vitro, Expressing, Staining